Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of Magnaporthe oryzae

Different external stimuli are perceived by multiple sensor histidine kinases and transmitted phosphorylation via the phosphotransfer protein Ypd1p in multistep phosphorelay system of high osmolarity glycerol signaling pathway filamentous fungi. How signal propagation takes place is still not known detail since kinase genes most fungi coded genome, whereas only one gene for exists. That raises hypothesis that various isoforms produced from a single sequence, perhaps alternative splicing, facilitating higher variability transduction. We found mRNA MoYPD1 rice blast fungus Magnaporthe oryzae subjected to an increased structural variation amplified putative on cDNA level. then generated mutant strains overexpressing these isoforms, purified products, present here previously unknown MoYpd1p isoform proteome Alternative splicing was be valid molecular mechanism increase diversity eukaryotic systems.